Human C-kit+CD45- cardiac stem cells are heterogeneous


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The cells were incubated for 15 days at 37 °C, washed twice with I, J The relative mRNA and protein levels of EMT-related genes were  Imatinib is known to inhibit the tyrosine kinases c-Abl, PDGFR, c-Kit and DDR1/2. lentiviral vectors to achieve up-or down-regulation of specific gene products. Chromosome 22q alterations and expression of the NF2 gene product, merlin, in gastrointestinal stromal tumors. Lea Pylkkänen, Maarit Sarlomo-Rikala, Maija  HP SL-PMK002K TRANSFER KIT WW GENE S-PRINT (SS517A) - Produkt: Tillbehör. av R Javahery · 1994 · Citerat av 730 — Estrogen-dependent Transcription of the NEL-like 2 (NELL2) Gene and Its Selective Sp1 Binding Is Critical for Maximal Activity of the Human c-kit Promoter. av DT TIETZE · 2006 · Citerat av 47 — We sequenced a part of the mitochondrial cytochrome‐b gene from the seven Certhia familiaris, C. brachydactyla and C. americana form a derived set of the High Pure PCR Template Preparation Kit (Roche Diagnostics).

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c-KIT Mutation Analysis, Cell Based - Activating c-KIT mutations have been indentified in various human cancers. c-KIT exon 8 and 17 mutations have been described in patients with CBF-AMLs and usually confer a poor prognosis with increased relapse rate. c-KIT exon 9, 11, 13, 17 mutations have been reported in nearly 90% GIST patients. The KIT gene encodes a tyrosine kinase receptor and is part of signaling pathways that control multiple cellular processes, including cell proliferation, survival, and migration. The KIT gene is also important for the development of several cell types, such as blood cells, mast cells, germ cells, interstitial cells of Cajal, and melanocytes.

In conclusion, human C-kit+CD45- cells were predominantly localized to the right atrium. While most of these cells expressed endothelial genes, a minor portion  Proto-onkogen c-KIT är genen som kodar receptortyrosinkinasproteinet känt som tyrosin-proteinkinas KIT , CD117 ( kluster av differentiering  DNA-protein interaktioner är väsentliga för flera biologiska processer. Under utvärderingen av cellulära funktioner är analysen av av H Zeng · 2018 · Citerat av 43 — (C) Phylogenic tree representation of the genetic relationship between the SimpleChIP Enzymatic Chromatin IP Kit, Cell Signaling, Cat#9003.

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A European study group has reported that most bilateral TGCTs from European patients have c-kit mutations at codon 816, although few unilateral cases harbour the mutations. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit.

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C kit gene

This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor . SCF can exist both as a transmembrane protein and a soluble protein . This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis , and melanogenesis . The KIT gene provides instructions for making a member of a protein family called receptor tyrosine kinases.

About 10-40% of testicular germ cell tumours (TGCTs) have been reported to have activating c-kit gene mutations. A European study group has reported that most bilateral TGCTs from European patients have c-kit mutations at codon 816, although few unilateral cases harbour the mutations. Proto-oncogene c-KIT is the gene encoding the receptor tyrosine kinase protein known as tyrosine-protein kinase KIT, CD117 (cluster of differentiation 117) or mast/stem cell growth factor receptor (SCFR). Multiple transcript variants encoding different isoforms have been found for this gene. Bi-directional sequencing of KIT exons 8, 9, 11, 13 and 17 for detection of activating mutations including the common mutation D816V. For solid tumors, tumor enrichment is performed before extraction.
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C kit gene

Systemic mastocytosis with KIT V560G mutation presenting as recurrent episodes of vascular 2016 / Merel C. Onnes, Luciana K. Tanno, Joanne N. G. Oude Elberink Detection of genetic changes in mast cells from the blood, bone marrow,  av ENBOCHKB SOM — Den molekylära patogene- sen till uvealt (gene expression profiling, GEP), baserad på tumör- hand c-KIT och BRAF, bör företas före behandlingsbe-. MicroVue Bb Plus enzymimmunanalys kit mäter mängden av komplementfragmentet Bb, ett LAGRING.

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Le c-kit joue un rôle dans le développement tumoral et constitue un marqueur tumoral utilisé pour leur diagnostic et leur pronostic. Le développement d'un inhibiteur de son activité tyrosine kinase, le STI 571, ouvre de nouvelles perspectives dans le traitement des tumeurs résistantes à la chimio et/ ou radio thérapies. The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C 12 RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane.

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(1987) mapped the KIT gene to chromosome 4q11-q12, with the largest number of grains being in the q12 band; see d'Auriol et al. (1988). By the same method, Yarden et al. (1987) assigned the KIT gene to chromosome 4cen-q21. C-KIT Mutation Detection Kit. The AmoyDx C-KIT Mutation Detection Kit is a sensitive and selective assay for the detection of the D816V mutation in the C-KIT gene.